In PAS-D staining, what is the primary purpose of including diastase?

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Multiple Choice

In PAS-D staining, what is the primary purpose of including diastase?

Explanation:
Diastase digestion in PAS-D staining serves to break down glycogen. Glycogen is PAS-positive, so without diastase it would contribute color everywhere glycogen is present. By digesting glycogen, the PAS reaction is removed from those areas, so any remaining staining after diastase comes from non-glycogen substances. This distinction allows you to tell glycogen apart from other PAS-positive materials, such as certain mucosubstances or fungal cell walls.

Diastase digestion in PAS-D staining serves to break down glycogen. Glycogen is PAS-positive, so without diastase it would contribute color everywhere glycogen is present. By digesting glycogen, the PAS reaction is removed from those areas, so any remaining staining after diastase comes from non-glycogen substances. This distinction allows you to tell glycogen apart from other PAS-positive materials, such as certain mucosubstances or fungal cell walls.

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